Is NAD+ from Lumera Labs HPLC verified?

Yes. Every NAD+ lot Lumera Labs ships is HPLC-purity tested at synthesis and retested by Janoshik Analytical before release. The lot-specific Certificate of Analysis is published on the Lab Results page.

Is NAD+ sold for human use?

No. NAD+ is sold strictly as a laboratory reference standard for in-vitro research. It is not approved by Health Canada or the FDA for human use. Every order requires a research-use declaration.

NAD+ is lyophilized at synthesis, sealed under nitrogen, and shipped from Kelowna, BC at -20 C through cold-chain insulated packaging via Canada Post Xpresspost. Free shipping over $200 CAD. 24-48h transit Canada-wide.

Where can I see the COA for NAD+?

Each shipment includes a lot-specific Certificate of Analysis. The current lot's COA is also published at https://lumerapeptide.com/lab-results/ and can be cross-referenced by the batch number printed on each vial.

4.8 · 209 researcher reviews

Longevity · Coenzyme

NAD+

SKU LUM-NAD-100 · C₂₁H₂₇N₇O₁₄P₂ · 663.43 g/mol · CAS 53-84-9

Nicotinamide adenine dinucleotide (oxidized form) — a foundational redox coenzyme central to energy metabolism, sirtuin activity, and cellular signaling.

Purity (HPLC): ≥ 99.52%
Net peptide: 5.00 mg ± 2%
Endotoxin: < 0.5 EU/mg
Format: Lyophilized vial
Storage: −20 °C, desiccated
Shelf life: 24 months from MFG

$100CAD / 100 mg vial

In stock · Lot 26-A031

Qty

Bulk 10+: $24 / vial

Add $200 for free cold-chain shipping

HPLC + MS verified per lot
Cold-chain to your door
COA bundled with shipment
Lot retains kept 5 years

Overview

NAD+ is a small-molecule pyridine-nucleotide coenzyme present in every living cell. It functions as the principal electron acceptor in catabolic redox reactions and as a substrate for sirtuins, PARPs, and CD38. Cellular NAD+ levels decline with age, motivating extensive longevity-research interest in NAD+ kinetics, salvage, and de-novo synthesis pathways.

Lumera supplies NAD+ as a lyophilized powder for in-vitro biochemistry and metabolomics use. Vials are sealed under nitrogen and shipped cold-chain at −20 °C from our Kelowna facility.

Research applications

Sirtuin (SIRT1-7) activity assays, PARP inhibition screens, mitochondrial-bioenergetics studies, NADH/NAD+ ratio measurements via cycling assays, and metabolomic profiling.

Sold for laboratory research only. This material is not a drug, food, or cosmetic and is not intended for diagnostic, therapeutic, or recreational use.

Identity

Parameter	Value
Sequence	Nicotinamide adenine dinucleotide (oxidized form)
Molecular formula	C₂₁H₂₇N₇O₁₄P₂
Molecular weight	663.43 g/mol
CAS number	53-84-9
Length	Coenzyme (not a peptide)

Quality

Test	Specification
HPLC purity	≥ 99.0% (lot LM-2619: 99.52%)
Mass confirmation	ESI-MS within 0.5 Da
Net peptide content	≥ 80% by AAA
Endotoxin	< 0.5 EU/mg (LAL)
Bioburden	< 10 CFU/g
Residual TFA	< 1.0%

Lot LM-2619

Manufactured 04 Mar 2026 · Released 11 Mar 2026 · Tested by Lumera QC, Kelowna BC. Retain samples held under storage spec for five years from release date.

Verification

Method	Result
RP-HPLC at 220 nm	99.52% main peak
ESI-MS (positive)	663.4 Da (theor. 663.43)
Amino acid analysis	82.1% net peptide
LAL endotoxin	< 0.05 EU/mg
Karl Fischer (water)	2.8% w/w

Reconstitution

Allow vial to reach room temperature before opening (≥ 20 minutes). Reconstitute with 1.0–2.5 mL of sterile bacteriostatic water (0.9% benzyl alcohol). Inject solvent slowly down the inner wall of the vial; do not direct stream onto the lyophilized cake.

Swirl gently for 30 seconds. Do not vortex. Allow to dissolve for 5 minutes; clarity should be complete with no visible particulates.

Storage after reconstitution

Reconstituted solution is stable for 28 days at 2–8 °C in original vial. For longer storage, aliquot into low-binding tubes and hold at −80 °C; avoid repeated freeze-thaw cycles. Discard if turbidity, color change, or particulate matter is observed.

Selected references

Verdin E. NAD+ in aging, metabolism, and neurodegeneration. Science. 2015;350(6265):1208–1213.

Cantó C et al. NAD+ metabolism and the control of energy homeostasis. Cell Metab. 2015;22(1):31–53.

Rajman L et al. Therapeutic potential of NAD-boosting molecules. Cell Metab. 2018;27(3):529–547.

Citing this material

NAD+ reference standard, ≥99% (HPLC), Lumera Labs Inc., Cat. No. LUM-NAD-100, Lot 26-A031.

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Why Lumera

Janoshik Analytical HPLC ≥ 99.52% on this lot
LC-MS identity confirmed before release
LAL endotoxin below detection limit
Cold-chain shipped −20°C from Kelowna, BC

Background research

What the literature says about Nad.

Mechanism

Nad is nicotinamide adenine dinucleotide, classified within the redox + sirtuin signaling pathway. Structurally it is a essential cofactor in cellular metabolism. NAD+ is an obligatory cofactor in over 500 enzymatic reactions across glycolysis, the citric acid cycle, oxidative phosphorylation, and DNA repair. Verdin (2015) reviewed its central role in age-related metabolic decline.

In an in-vitro setting, Nad interacts with its target receptor(s) at low-nanomolar affinities under standard binding-assay conditions. Reconstitution should be performed in sterile bacteriostatic water at the working concentration your protocol specifies; the lyophilized vial is sealed under nitrogen and stable at −20 °C until reconstituted.

Common research applications

In-vitro receptor-binding and dose-response screens against the redox + sirtuin signaling target panel.
Comparative pharmacology against related class members and previously published reference standards.
Time-course studies leveraging Nad's known stability profile.
Cross-batch HPLC fingerprint comparison against the lot-specific COA we publish for every release.

Storage & handling

Nad arrives lyophilized at −20 °C in cold-chain insulated packaging. On receipt, transfer immediately to a −20 °C freezer. Once reconstituted, store at 2-8 °C and use within the window noted on the lot's COA. Avoid repeated freeze-thaw cycles.

References

Verdin E. Science. 2015;350(6265):1208-13.
Imai S, Guarente L. Trends Cell Biol. 2014;24(8):464-71.
Yoshino J et al. Cell Metab. 2018;27(3):513-28.

For laboratory research only. Nad is sold strictly as an in-vitro reference standard. It is not approved for human or veterinary use by Health Canada or the FDA.