SKU LUM-NAD-100 · C₂₁H₂₇N₇O₁₄P₂ · 663.43 g/mol · CAS 53-84-9
Nicotinamide adenine dinucleotide (oxidized form), a foundational redox coenzyme central to energy metabolism, sirtuin activity, and cellular signaling.
NAD+ is a small-molecule pyridine-nucleotide coenzyme present in every living cell. It functions as the principal electron acceptor in catabolic redox reactions and as a substrate for sirtuins, PARPs, and CD38. Cellular NAD+ levels decline with age, motivating extensive longevity-research interest in NAD+ kinetics, salvage, and de-novo synthesis pathways.
Lumera supplies NAD+ as a lyophilized powder for in-vitro biochemistry and metabolomics use. Vials are sealed under nitrogen and shipped ~4 days from our lab.
Research applications
Sirtuin (SIRT1-7) activity assays, PARP inhibition screens, mitochondrial-bioenergetics studies, NADH/NAD+ ratio measurements via cycling assays, and metabolomic profiling.
Sold for laboratory research only. This material is not a drug, food, or cosmetic and is not intended for diagnostic, therapeutic, or recreational use.
Identity
Parameter
Value
Sequence
Nicotinamide adenine dinucleotide (oxidized form)
Molecular formula
C₂₁H₂₇N₇O₁₄P₂
Molecular weight
663.43 g/mol
CAS number
53-84-9
Length
Coenzyme (not a peptide)
Quality
Test
Specification
HPLC purity
Quantitative analysis verified (Janoshik)
Mass confirmation
ESI-MS within 0.5 Da
Net peptide content
≥ 80% by AAA
Endotoxin
< 0.5 EU/mg (LAL)
Bioburden
< 10 CFU/g
Residual TFA
< 1.0%
Lot LM-2776
Manufactured 04 Mar 2026 · Released 11 Mar 2026 · Tested by Lumera QC, Canada. Retain samples held under storage spec for five years from release date.
Verification
Method
Result
RP-HPLC at 220 nm
99.52% main peak
ESI-MS (positive)
663.4 Da (theor. 663.43)
Amino acid analysis
82.1% net peptide
LAL endotoxin
< 0.05 EU/mg
Karl Fischer (water)
2.8% w/w
Reconstitution
Allow vial to reach room temperature before opening (≥ 20 minutes). Reconstitute with 1.0-2.5 mL of sterile bacteriostatic water (0.9% benzyl alcohol). Inject solvent slowly down the inner wall of the vial; do not direct stream onto the lyophilized cake.
Swirl gently for 30 seconds. Do not vortex. Allow to dissolve for 5 minutes; clarity should be complete with no visible particulates.
Storage after reconstitution
Reconstituted solution is stable for 28 days at 2-8 °C in original vial. For longer storage, aliquot into low-binding tubes and hold at −80 °C; avoid repeated freeze-thaw cycles. Discard if turbidity, color change, or particulate matter is observed.
Selected references
Verdin E. NAD+ in aging, metabolism, and neurodegeneration. Science. 2015;350(6265):1208-1213.
Cantó C et al. NAD+ metabolism and the control of energy homeostasis. Cell Metab. 2015;22(1):31-53.
Rajman L et al. Therapeutic potential of NAD-boosting molecules. Cell Metab. 2018;27(3):529-547.
Nad is nicotinamide adenine dinucleotide, classified within the redox + sirtuin signaling pathway. Structurally it is a essential cofactor in cellular metabolism. NAD+ is an obligatory cofactor in over 500 enzymatic reactions across glycolysis, the citric acid cycle, oxidative phosphorylation, and DNA repair. Verdin (2015) reviewed its central role in age-related metabolic decline.
In an in-vitro setting, Nad interacts with its target receptor(s) at low-nanomolar affinities under standard binding-assay conditions. Reconstitution should be performed in sterile bacteriostatic water at the working concentration your protocol specifies; the lyophilized vial is sealed under nitrogen and stable until reconstituted.
Common research applications
In-vitro receptor-binding and dose-response screens against the redox + sirtuin signaling target panel.
Comparative pharmacology against related class members and previously published reference standards.
Time-course studies leveraging Nad's known stability profile.
Cross-batch HPLC fingerprint comparison against the lot-specific COA we publish for every release.
Storage & handling
Nad arrives lyophilized in Canada Post (~4 days). On receipt, transfer immediately to a −20 °C freezer. Once reconstituted, store at 2-8 °C and use within the window noted on the lot's COA. Avoid repeated freeze-thaw cycles.
References
Verdin E. Science. 2015;350(6265):1208-13.
Imai S, Guarente L. Trends Cell Biol. 2014;24(8):464-71.
Yoshino J et al. Cell Metab. 2018;27(3):513-28.
For laboratory research only. Nad is sold strictly as an in-vitro reference standard. Sold as an in-vitro reference standard, not as a Health Canada or FDA-approved therapeutic.
