MOTS-c (mitochondrial open reading frame of the 12S rRNA-c) is a 16-residue peptide encoded within the mitochondrial genome and translated in the cytoplasm. It activates AMPK, modulates folate-methionine cycling, and is investigated for its roles in glucose homeostasis, exercise capacity, and longevity pathways.
Each vial is lyophilized from acetate buffer, sealed under nitrogen, and shipped ~4 days from our lab.
Research applications
AMPK-activation screens (Thr172 phosphorylation), 5-methyl-THF/SAM metabolomics, in-vitro glucose-uptake assays in skeletal myoblasts, and comparative mitochondrial-derived peptide pharmacology.
Sold for laboratory research only. This material is not a drug, food, or cosmetic and is not intended for diagnostic, therapeutic, or recreational use.
Identity
Parameter
Value
Sequence
MRWQEMGYIFYPRKLR
Molecular formula
C₁₀₀H₁₅₈N₂₆O₂₂S
Molecular weight
2174.55 g/mol
CAS number
1627580-64-6
Length
16 residues
Quality
Test
Specification
HPLC purity
99.443%
Mass confirmation
ESI-MS within 0.5 Da
Net peptide content
≥ 80% by AAA
Endotoxin
< 0.5 EU/mg (LAL)
Bioburden
< 10 CFU/g
Residual TFA
< 1.0%
Lot LM-2774
Manufactured 04 Mar 2026 · Released 11 Mar 2026 · Tested by Lumera QC, Canada. Retain samples held under storage spec for five years from release date.
Verification
Method
Result
RP-HPLC at 220 nm
99.13% main peak
ESI-MS (positive)
2174.6 Da (theor. 2174.55)
Amino acid analysis
82.1% net peptide
LAL endotoxin
< 0.05 EU/mg
Karl Fischer (water)
2.8% w/w
Reconstitution
Allow vial to reach room temperature before opening (≥ 20 minutes). Reconstitute with 1.0-2.5 mL of sterile bacteriostatic water (0.9% benzyl alcohol). Inject solvent slowly down the inner wall of the vial; do not direct stream onto the lyophilized cake.
Swirl gently for 30 seconds. Do not vortex. Allow to dissolve for 5 minutes; clarity should be complete with no visible particulates.
Storage after reconstitution
Reconstituted solution is stable for 28 days at 2-8 °C in original vial. For longer storage, aliquot into low-binding tubes and hold at −80 °C; avoid repeated freeze-thaw cycles. Discard if turbidity, color change, or particulate matter is observed.
Selected references
Lee C et al. The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis. Cell Metab. 2015;21(3):443-454.
Reynolds JC et al. MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis. Nat Commun. 2021;12(1):470.
Kim KH et al. The mitochondrial-encoded peptide MOTS-c translocates to the nucleus to regulate nuclear gene expression. Cell Metab. 2018;28(3):516-524.
Mots C is a mitochondrial-derived peptide, classified within the mitochondrial-nuclear signaling pathway. Structurally it is a 16-residue MDP from MT-RNR1. MOTS-c is a 16-residue mitochondrial-derived peptide encoded within the 12S rRNA gene. Lee et al. (2015) demonstrated its role in metabolic homeostasis through AMPK activation.
In an in-vitro setting, Mots C interacts with its target receptor(s) at low-nanomolar affinities under standard binding-assay conditions. Reconstitution should be performed in sterile bacteriostatic water at the working concentration your protocol specifies; the lyophilized vial is sealed under nitrogen and stable until reconstituted.
Common research applications
In-vitro receptor-binding and dose-response screens against the mitochondrial-nuclear signaling target panel.
Comparative pharmacology against related class members and previously published reference standards.
Time-course studies leveraging Mots C's known stability profile.
Cross-batch HPLC fingerprint comparison against the lot-specific COA we publish for every release.
Storage & handling
Mots C arrives lyophilized in Canada Post (~4 days). On receipt, transfer immediately to a −20 °C freezer. Once reconstituted, store at 2-8 °C and use within the window noted on the lot's COA. Avoid repeated freeze-thaw cycles.
References
Lee C et al. Cell Metab. 2015;21(3):443-54.
Reynolds JC et al. Nat Commun. 2021;12:470.
Yen K et al. Trends Endocrinol Metab. 2013;24(5):222-8.
For laboratory research only. Mots C is sold strictly as an in-vitro reference standard. Sold as an in-vitro reference standard, not as a Health Canada or FDA-approved therapeutic.
