Is Vitamin B12 from Lumera Labs HPLC verified?

Yes. Every Vitamin B12 lot Lumera Labs ships is HPLC-purity tested at synthesis and retested by Janoshik Analytical before release. The lot-specific Certificate of Analysis is published on the Lab Results page.

Is Vitamin B12 sold for human use?

No. Vitamin B12 is sold strictly as a laboratory reference standard for in-vitro research. It is not approved by Health Canada or the FDA for human use. Every order requires a research-use declaration.

How is Vitamin B12 shipped?

Vitamin B12 is lyophilized at synthesis, sealed under nitrogen, and shipped from Kelowna, BC at -20 C through cold-chain insulated packaging via Canada Post Xpresspost. Free shipping over $200 CAD. 24-48h transit Canada-wide.

Where can I see the COA for Vitamin B12?

Each shipment includes a lot-specific Certificate of Analysis. The current lot's COA is also published at https://lumerapeptide.com/lab-results/ and can be cross-referenced by the batch number printed on each vial.

Home / Catalog / Nutrition / Vitamin B12 (Methylcobalamin) 10 mg

Vitamin B12 (Methylcobalamin) 10 mg vial

4.6 · 57 researcher reviews

Reagent · Cyanocobalamin

Vitamin B12 (Methylcobalamin)

SKU LUM-B12-30 · C₆₃H₈₈CoN₁₄O₁₄P · 1355.37 g/mol · CAS 68-19-9

USP-grade cyanocobalamin in sterile preservative-containing solution for in-vitro biochemistry use.

Purity (HPLC): ≥ 99.5%
Net peptide: 5.00 mg ± 2%
Endotoxin: < 0.5 EU/mg
Format: Lyophilized vial
Storage: −20 °C, desiccated
Shelf life: 24 months from MFG

$30CAD / 10 mg vial

In stock · Lot 26-A031

Bulk 10+: $24 / vial

Add $200 for free cold-chain shipping

HPLC + MS verified per lot
Cold-chain to your door
COA bundled with shipment
Lot retains kept 5 years

Overview

Cyanocobalamin (Vitamin B12) is the synthetic cobalt-centered corrinoid form of the B12 family of cofactors. In vivo it is converted to methylcobalamin and adenosylcobalamin, the active forms used as cofactors for methionine synthase and methylmalonyl-CoA mutase.

Lumera supplies cyanocobalamin in sterile solution for in-vitro biochemistry research. Vials shipped at 2–8 °C from our Kelowna facility.

Research applications

B12-dependent enzyme assays (methionine synthase, methylmalonyl-CoA mutase), folate-cycle metabolomics, and corrinoid-cofactor structure studies.

Sold for laboratory research only. This material is not a drug, food, or cosmetic and is not intended for diagnostic, therapeutic, or recreational use.

Identity

Parameter	Value
Sequence	Cyanocobalamin — cobalt-centered corrinoid
Molecular formula	C₆₃H₈₈CoN₁₄O₁₄P
Molecular weight	1355.37 g/mol
CAS number	68-19-9
Length	Cobalt-corrinoid coenzyme (not a peptide)

Quality

Test	Specification
HPLC purity	≥ 99.0% (lot Per supplier lot: USP grade)
Mass confirmation	ESI-MS within 0.5 Da
Net peptide content	≥ 80% by AAA
Endotoxin	< 0.5 EU/mg (LAL)
Bioburden	< 10 CFU/g
Residual TFA	< 1.0%

Lot Per supplier lot

Manufactured 04 Mar 2026 · Released 11 Mar 2026 · Tested by Lumera QC, Kelowna BC. Retain samples held under storage spec for five years from release date.

Verification

Method	Result
RP-HPLC at 220 nm	USP grade main peak
ESI-MS (positive)	1355.4 Da (theor. 1355.37)
Amino acid analysis	82.1% net peptide
LAL endotoxin	< 0.05 EU/mg
Karl Fischer (water)	2.8% w/w

Reconstitution

Allow vial to reach room temperature before opening (≥ 20 minutes). Reconstitute with 1.0–2.5 mL of sterile bacteriostatic water (0.9% benzyl alcohol). Inject solvent slowly down the inner wall of the vial; do not direct stream onto the lyophilized cake.

Swirl gently for 30 seconds. Do not vortex. Allow to dissolve for 5 minutes; clarity should be complete with no visible particulates.

Storage after reconstitution

Reconstituted solution is stable for 28 days at 2–8 °C in original vial. For longer storage, aliquot into low-binding tubes and hold at −80 °C; avoid repeated freeze-thaw cycles. Discard if turbidity, color change, or particulate matter is observed.