SKU LUM-B12-30 · C₆₃H₈₈CoN₁₄O₁₄P · 1355.37 g/mol · CAS 68-19-9
USP-grade cyanocobalamin in sterile preservative-containing solution for in-vitro biochemistry use.
Purity (HPLC)
≥ 99.5%
Net peptide
5.00 mg ± 2%
Endotoxin
< 0.5 EU/mg
Format
Lyophilized vial
Storage
−20 °C, desiccated
Shelf life
24 months from MFG
$35CAD/ 10 mg vial
In stock · Lot LM-2755
Qty
Add $300 for Free shipping
HPLC + MS verified per lot
~4 days to your door
COA bundled with shipment
Lot samples retained 5 years
Overview
Cyanocobalamin (Vitamin B12) is the synthetic cobalt-centered corrinoid form of the B12 family of cofactors. In vivo it is converted to methylcobalamin and adenosylcobalamin, the active forms used as cofactors for methionine synthase and methylmalonyl-CoA mutase.
Lumera supplies cyanocobalamin in sterile solution for in-vitro biochemistry research. Vials shipped at 2–8 °C from our lab.
Sold for laboratory research only. This material is not a drug, food, or cosmetic and is not intended for diagnostic, therapeutic, or recreational use.
Identity
Parameter
Value
Sequence
Cyanocobalamin — cobalt-centered corrinoid
Molecular formula
C₆₃H₈₈CoN₁₄O₁₄P
Molecular weight
1355.37 g/mol
CAS number
68-19-9
Length
Cobalt-corrinoid coenzyme (not a peptide)
Quality
Test
Specification
HPLC purity
USP grade
Mass confirmation
ESI-MS within 0.5 Da
Net peptide content
≥ 80% by AAA
Endotoxin
< 0.5 EU/mg (LAL)
Bioburden
< 10 CFU/g
Residual TFA
< 1.0%
Lot LM-2755
Manufactured 04 Mar 2026 · Released 11 Mar 2026 · Tested by Lumera QC, Canada. Retain samples held under storage spec for five years from release date.
Verification
Method
Result
RP-HPLC at 220 nm
USP grade main peak
ESI-MS (positive)
1355.4 Da (theor. 1355.37)
Amino acid analysis
82.1% net peptide
LAL endotoxin
< 0.05 EU/mg
Karl Fischer (water)
2.8% w/w
Reconstitution
Allow vial to reach room temperature before opening (≥ 20 minutes). Reconstitute with 1.0–2.5 mL of sterile bacteriostatic water (0.9% benzyl alcohol). Inject solvent slowly down the inner wall of the vial; do not direct stream onto the lyophilized cake.
Swirl gently for 30 seconds. Do not vortex. Allow to dissolve for 5 minutes; clarity should be complete with no visible particulates.
Storage after reconstitution
Reconstituted solution is stable for 28 days at 2–8 °C in original vial. For longer storage, aliquot into low-binding tubes and hold at −80 °C; avoid repeated freeze-thaw cycles. Discard if turbidity, color change, or particulate matter is observed.
Selected references
Banerjee R, Ragsdale SW. The many faces of vitamin B12. Annu Rev Biochem. 2003;72:209–247.
Green R et al. Vitamin B12 deficiency. Nat Rev Dis Primers. 2017;3:17040.
Hannibal L et al. Biomarkers and algorithms for the diagnosis of vitamin B12 deficiency. Front Mol Biosci. 2016;3:27.
