How much bac water for 500 mg NAD+?
Concentration and U-100 syringe unit chart for a 500 mg vial. Measurement arithmetic for preparing research solutions. For laboratory research use only.
A common target is 5 mL of bacteriostatic water (total) for a 500 mg NAD+ vial, in a vial that can hold it, giving 100 mg/mL, so 10 units on a U-100 insulin syringe (0.1 mL) holds 10 mg. Alternatives: 2.5 mL gives 200 mg/mL; 3 mL gives 166.67 mg/mL; 10 mL gives 50 mg/mL. Less water gives a stronger solution and fewer units per draw; more water gives finer resolution per unit.
Check your vial's capacity first. A standard 3 mL peptide vial cannot hold 5 mL. Add only what the vial can safely hold (leave headspace), let the powder dissolve fully, then transfer the solution to a larger sterile vial and bring it to the final volume, or divide it into labelled aliquots. Very concentrated NAD+ solutions dissolve slowly; if powder remains after gentle swirling, more diluent is needed.
500 mg NAD+ reconstitution chart
Concentration = 500 mg divided by the mL of water added. On a U-100 insulin syringe, 100 units = 1 mL, so 10 units = 0.1 mL. Units for a given amount = amount / concentration x 100.
| Bac water added | Concentration | In 10 units (0.1 mL) | Units for 10 mg | Units for 25 mg | Units for 50 mg | Units for 100 mg |
|---|---|---|---|---|---|---|
| 2.5 mL | 200 mg/mL | 20 mg | 5 | 12.5 | 25 | 50 |
| 3 mL | 166.67 mg/mL | 16.67 mg | 6 | 15 | 30 | 60 |
| 5 mL | 100 mg/mL | 10 mg | 10 | 25 | 50 | 100 |
| 10 mL | 50 mg/mL | 5 mg | 20 | 50 | 100 | 200* |
Highlighted row = the common default for this vial. Unit values are on a U-100 insulin syringe (100 units = 1 mL), rounded to 0.1 unit; read to the nearest graduation your syringe has. * More than 100 units means more than 1 mL, beyond a standard 1 mL insulin syringe: use a more concentrated mix (less water) or a larger graduated syringe.
How to reconstitute 500 mg NAD+
- Equilibrate. Let the sealed vial and the bacteriostatic water reach room temperature (about 15 to 20 minutes) to limit condensation and help the powder dissolve.
- Swab both stoppers. Wipe the rubber stopper of the peptide vial and of the water vial with a fresh alcohol swab and let them air-dry.
- Measure the water. Draw 5 mL of bacteriostatic water (or your chosen volume from the chart) with a sterile syringe.
- Add it down the vial wall. Insert the needle through the stopper at an angle and release the water slowly down the inside wall of the vial, not directly onto the powder.
- Swirl, do not shake. Gently swirl or roll the vial until the powder is fully dissolved and the solution is clear. Allow a few minutes; some peptides dissolve slowly.
- Label it. Write the date and the concentration (100 mg/mL at the default volume) on the vial.
- Refrigerate. Store the reconstituted vial at 2 to 8 °C, protected from light.
More detail: bacteriostatic vs sterile water and reading insulin syringes.
Storage after reconstitution
- Keep the reconstituted vial at 2 to 8 °C (refrigerator, not the door), protected from light.
- Lumera's product pages list 28 days at 2 to 8 °C in the original vial; follow the window on the lot's COA. Bacteriostatic water's benzyl alcohol limits microbial growth across repeated stopper punctures; sterile water does not.
- Do not refreeze the working vial. For longer holding, divide into aliquots once and freeze, avoiding repeated freeze-thaw cycles.
- Unopened lyophilized vials keep best frozen (-20 °C). See the peptide storage temperature guide.
Troubleshooting
The solution is cloudy
Give it time: let the vial stand in the fridge for 15 to 30 minutes and swirl gently again. If cloudiness, particles or a colour change persist after the powder has dissolved, do not use the solution for quantitative work; discard it.
Foam or bubbles
Usually caused by shaking or by streaming water onto the powder. Let the vial rest upright in the fridge until the foam settles, and next time add the water slowly down the vial wall and swirl instead of shaking.
Left out at room temperature overnight
Degradation speeds up at room temperature, and how much is lost varies by peptide. Return the vial to 2 to 8 °C, note the excursion on the label, and for work where exact concentration matters, prepare a fresh vial.
NAD+ 500 mg
NAD+ 500 mg: $120 CAD per vial. A published Testides Certificate of Analysis for this vial size is on Lab Results.
Bacteriostatic water: 3 mL $10 CAD or 10 mL $15 CAD.
View NAD+ 500 mg Bacteriostatic water Peptide calculatorOther sizes
NAD+ reconstitution FAQ
How much bacteriostatic water do I add to 500 mg NAD+?
Most labs add 5 mL, which gives 100 mg/mL. Alternatives: 2.5 mL gives 200 mg/mL; 3 mL gives 166.67 mg/mL; 10 mL gives 50 mg/mL. The volume is a choice; what matters is recording the resulting concentration on the vial label.
How many units is 50 mg of NAD+ in a 5 mL mix?
At 100 mg/mL, 50 mg is in 50 units on a U-100 insulin syringe (0.5 mL). Units = amount / concentration x 100.
What concentration does 500 mg NAD+ make with 2 mL of water?
500 mg divided by 2 mL is 250 mg/mL, so each 10 units (0.1 mL) holds 25 mg.
How long does reconstituted NAD+ last in the fridge?
Lumera's product pages list reconstituted solutions as stable for 28 days at 2 to 8 °C in the original vial, protected from light. Use within the window noted on the lot's COA and discard if the solution turns cloudy, changes colour or shows particles.
Should I shake the NAD+ vial to dissolve it?
No. Swirl or roll the vial gently and let it stand a few minutes. Shaking creates foam and can degrade peptides.
All reconstitution charts by peptide · Peptide calculator for custom volumes.
Research use only. This page is measurement arithmetic for preparing research solutions from a lyophilized vial. It is not a dosing guide. Lumera Labs supplies peptides as laboratory reference standards; they are not approved drugs and are not intended for therapeutic use.